(Include all 5 Points)
- Identifythespecies,strains,ages,sex,andnumbersofanimalstobeusedintheproposed work.
- Justifytheuseofanimals,thechoiceofspecies,andthenumberstobeused.Ifanimalsarein short supply, costly, or to be used in large numbers, provide an additional rationale for their selection and numbers.
- Describetheproceduresforensuringthatdiscomfort,distress,pain,andinjurywillbelimitedto that which is unavoidable in the conduct of scientifically sound research. Describe the use of analgesic, anesthetic, and tranquilizing drugs and/or comfortable restraining devices, where appropriate, to minimize discomfort, distress, pain, and injury.
- Methodofeuthanasiatobeusedandthereasonsforitsselection.Statewhetherthismethod is consistent with the recommendations of the American Veterinary Medical Association (AVMA) Guidelines on Euthanasia. If not, include a scientific justification for not following the recommendations.